Fact Sheet
SMRV - Virus Testing
Interest Group
Pharmaceutical industry, media manufacturer, Research and Development, cell banks, cell line development.
Description
Two-stage test service for Squirrel Monkey Retrovirus (SMRV): cells are screened for SMRV DNA and only positive cell lines are subsequently examined for active virus by direct test (RT-PCR).
Recommended Sample Material
Cell cultures supernatants with cells.
Method
Conventional PCR and RT-PCR.
Applied PCR System
In-house.
Applied Extraction
QiaAMP DSP Virus Spin Kit (Qiagen AG), QiaAMP DSP Virus Kit (Qiagen AG) and ExtractNow DNA/RNA Kit (Minerva Biolabs GmbH).
Process Control
Extraction and Amplification.
Sample Volume / PCR
5 µl.
Average Turnaround Time
48 to 72 hours after sample receipt, express testing scheme* possible.
Sensitivity of Method
Based on sample matrix.
Regulatory Compliance
Please note that the sensitivity of the assay regarding your matrix may need to be validated. Please contact us for further information.
Sample Requirements
Order No.
Virus Testing ‘SMRV Screen’ 46-2001
Virus Testing ‘SMRV Direct Test’ 46-2002
Volume Discount Available
Yes, on request.
Additional Information
Please note: repetitions which are not caused by our laboratory and *express testing schemes are subject to additonal charges. Please contact us for further information.